Evolving Enzymes
Inovating IVD
Plasmid Extraction Mini Kit uses the spin column method to effectively extract high yields of plasmid DNA with high-purity. The isolated plasmid DNA can be used for various experiments such as sequencing, sub-cloning, and transformation, etc.
Support file
Specification:
Principle: mini spin column (silica matrix)
Sample size: 1 ~ 3 ml Component:
Size of plasmid or construct: < 15 kb
Operation time: < 25 minutes
Typical Yield: 20 ~ 30 µg
Binding capacity: 60 µg/ column
Column applicability: centrifugation and vaccum
Important Notes:
1. Store RNase A at -20 °C upon recipit of kit.
2. Add 0.5 ml of FAPD1 Buffer to a RNase A tube, Dissolve the RNase A by vortexing. Briefly spin the tube and transfer the total RNase A mixture back to the FAPD1 bottle, mix well by vortexing and store the FAPD1 buffer at 4 °C.
3. If precipitates have formed in FAPD2 Buffer, warm the buffer in 37°C waterbath to dissolve precipitates.
4. Preparation of W1 Buffer and Wash Buffer by adding 96 ~100% ethanol (not provided) for first use.
5. Centrifugation steps are done by a microcentrifuge capable of the speed at 11,000 ~1,8000 x g.
FAPD1 Buffer
FAPD2 Buffer
FAPD3 Buffer
W1 Buffer
Wash Buffer (concentrate)
Elution Buffer
FAPD Column
Collection Tube
RNase A
Procedure:
