GEL/ PCR Purification Kit contains a silica-gel-membrane for binding of DNA in high-salt buffer (pH 4.5~5.5) and elution of DNA with low-salt buffer (pH 7.0~9.0) or ddH2O. The purification procedure removes primers, nucleotides, enzyme, salts, ethidium bromide and agarose impurities from DNA samples.
Principle: spin column (silica matrix) DNA Binding capacity of spin column: 20 µg Sample size: up to 300 mg of agarose gel up to 100 µl of reaction solution DNA size: 65 bp ~ 10 kbp Recovery: 70% ~ 85% for Gel extraction 90% ~ 95% for PCR clean-up Operation time: 10 ~ 20 min Elution volume: 40 µl Important Notes: 1. Buffer provided in this kit contain irritants. Wear gloves and lab coat when handling these buffer. 2. Add the required volume of ethanol (96~100%) to Wash Buffer before use. 3. Centrifugation steps are done by a microcentrifuge capable of the speed at 11,000 ~1,8000 x g.
