DESCRIPTION

    TOROGreen^® One HS Taq Kit includes two components, 2× One HS Taq Mix and TOROGreen^® 6×DNA Loading Buffer. 2× One HS Taq Mix is a ready-to-use premixed solution containing hotstart Taq DNA polymerase, PCR buffer, dNTPs and PCR enhancer. This mix contains all components for PCR except primers and template DNA and shows specific and efficient amplification. TOROGreen^® 6×DNA loading buffer contains the fluorescence dye, which is directly detected on 470nm LED transilluminator after the DNA electrophoresis.

FEATURES

  - Enables greater PCR performance than conventional Taq DNA polymerase.

  -This reagent contains anti-Taq antibodies for hot start PCR. Hot start technology realizes highly specific and sensitive PCR.

  -Easy-to-use: offer TOROGreen^® 6×DNA loading buffer premixed with fluorescent dye for directly observed on 470nm LED transilluminator after electrophoresis.

APPLICATION

  -General PCR products for TA cloning.

  -Colony direct PCR

  -Multiplex PCR for SNPs etc.

  -High-throughput PCR.

COMPONENTS

The kit includes the following reagents, which can be used for 200 reactions for a total 50ul reaction volume, or 500 reactions for a total 20ul reaction volume.

     Cat No. ：TGO-100B

   2× One HS Taq Mix                                            1ml ×5tube

     TOROGreen^® 6×DNA loading buffer              1ml ×2 tube

  We also offer more small package as the following.

     Cat No. ：TGO-100S      

     2× One HS Taq Mix                                           0.5ml ×1tube

    TOROGreen^® 6×DNA loading buffer              0.2ml ×1 tube

PROTOCOL

1. Standard reaction setup

(1) All components should be completely thawed before preparing the mixture at room temperature.

3. Electrophoresis.

(1) Add 1 volume of TOROGreen^® 6×DNA loading buffer to 5 volumes of PCR products.

(2) Mix well, spin down and load the mixture and the suitable TOROGreen^® Loading Marker.

(3) Run on agarose electrophoresis to detect PCR products and marker. No additional dye is required for the PCR products and marker.

(4) Realtime observation of electrophoresis is also pos- sible if a 470nm blue light source is fitted to the electrophoresis tank.

(5) Use the 470nm blue-light transilluminator or imaging systems photograph the gel.

STORAGE

     This reagent can be stored at 4°C for 1 months. For longer storage,  this reagent should be kept at -20°C.