【Description】

• TOROBlue^® RT Kit for qPCR-FD- is a room-temperature stable RT kit to perform for qPCR. The engineered reverse transcriptase in this kit allows the synthesis of high-quality first-strand cDNA. 2×RT buffer for qPCR is  optimized for highly efficient synthesis of short-chain cDNAs suitable for qPCR. The protocol is simple, and the reaction can be completed in 10-25 min.

【Feature】

• -Room-Temperature stable: the performance is not easily decrease during during storing and shipping. 

• -Easy-to-use: add 50μL 2×RT buffer into a single tube to prepare the RT premix easily.

• -Maximum flexibility: cutting each tube allows for 5 or10 RT reactions.

• -High performance：an engineered reverse transcriptase allows the synthesis of high-quality cDNA.

【Components】

•  TOROBlue^® RT Kit for qPCR-FD- includes the following reagents, which can be used for 120 reactions for a total 20µL reaction volume or 240 reactions for a total 10µL reaction volume .

• NO.	Components	Size
  1	Freeze-dried RT PreMix-002-	8-tube strips×3
  2	2×RT buffer for qPCR	1.25mL/tube×1
  3	Water-DEPC treated	1.25mL/tube×1

• Notes: Freeze-dried RT PreMix-002 contains lyophilized  Reverse transcriptase, RNase Inhibitor and, Random hexamer and/or oligo(dT)20 primers; 2×RT buffer for qPCR contains reaction buffer optimized for highly efficient synthesis of short-chain cDNAs suitable for qPCR. 

【Storage】

•  Store the kit at 2-8℃ in a dry environment for 24 months.

【References】

•  Bustin SA, Benes V, Garson JA, etc,al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. ClinChem.2009,55(4):611-22.

【Application】

• [1] Xu K, Meng Z, Xian XM,et al.LncRNA PVT1 induces chondrocyte apoptosis through upregulation of TNF-α in synoviocytes by sponging miR-211-3p[J].Molecular and Cellular Probes, 2020, 52:101560.

• [2]  Su X, Zhu Y, Hou Y,et al.PVT1 induces NSCLC cell migration and invasion by regulating IL-6 viasponging miR-760.[J].Molecular and cellular probes, 2020, 54:101652.

• [3] Xia CP, Pan T, Zhang N,et al.Sp1 promotes dental pulp stem cell osteoblastic differentiation through regulating noggin[J]. Molecular and cellular probes, 2020,  50:101504.

• [4] MY Xie, LH Yang, JY Cheng,et al.Gracillin relieves pulmonary fibrosis by suppressing the STAT3 axis[J].Journal of Ethnopharmacology,2023,316:116704,

• [5] Wang Y, Fan J, Tong Y,et al. Bioinformatics analysis of ferroptosis-related gene AKR1C3 as a potential biomarker of asthma and its identification in BEAS-2B cells. Comput Biol Med. 2023 ,3(158):106740.

• [6] Wen J, Jiang F, Liu M,et al.Identification and expression analysis of Cathepsin B-like protease 2 genes in tomato at abiotic stresses especially at High temperature[J]. Scientia Horticulturae, 2021, 277(10):109799.

【Documents】

•     Manuals:   TOROBlue^® RT Kit for qPCR -FD-(RTQ-108) -English-

•     Brochures:   Real-time PCR Premix for gene expression analysis

•     MSDS： Material Safety Data Sheet for RTQ-108

•     COA： Certificate of Analysis for RTQ-108  

    

【Order information】